Determination of Disulfide Bonds in γ-46 Gliadin

—The disulfide bonds in γ-46 gliadin were identified: Cys 173 –Cys 192 , Cys 212 –Cys 291 , Cys 165 –Cys 199 (or Cys 200 ), Cys 283 –Cys 200 (or Cys 199 ). The disulfide-containing peptides were obtained by limited hydrolysis of the intact protein with chymotrypsin at an enzyme/substrate ratio of 1:1000 at 20°C for 22 h with subsequent digestion of disulfide-containing fragments with trypsin and chymotrypsin. The locations of disulfide bonds were determined by sequencing disulfide-containing fractions and constituent peptides and comparison of the obtained sequences with the partial amino acid sequence of γ-46 gliadin determined earlier.